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. 2010 Jun 10;6(6):e1000986. doi: 10.1371/journal.pgen.1000986

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Vrbsky et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Northern analysis of small RNAs from wild-type and the indicated RdDM mutants. The membrane was hybridized with a CCCTAAA probe, stripped and rehybridized with the TTTAGGG probe. Electronically merged autoradiograms show faster migration of the C-siRNA that is due to a sequence bias towards pyrimidines. The loading control represents a large RNA that hybridizes to the TTTAGGG probe. (B) Distribution of siRNAs containing at least 12 nt of telomeric sequence in different Argonaute complexes. (C) Distribution of AGO4-associated C- and G-siRNAs according to the extent of homology to telomeric sequence. The total number of siRNAs is indicated on the y-axis.