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. 2010 Jun 10;6(6):e1000986. doi: 10.1371/journal.pgen.1000986

Figure 7. Telomeric heterochromatin in RdDM mutants.

Figure 7

(A) Representative ChIP data of wild-type and rdr2 plants. Chromatin was immunoprecipitated with antibodies against histone H3, H3K4me3, H3K9me2 and H3K27me1, blotted onto a membrane and hybridized with the TTTAGGG probe. The same membrane was stripped after exposure and rehybridized with the centromeric CEN180 probe. Data from three independent ChIP experiments were used for quantification. Signals were normalized to mock. (B) Analysis of ChIP fractions from wild-type and rdr2 plants by PCR with primers spanning the 1L-0 locus. (C) qPCR analysis of H3K4me3, H3K9me2 and H3K27me1 at the 1L-0 locus in wild-type and rdr2 plants. Each value represents an average of three qPCR measurements normalized to histone H3 occupancy for each ChIP fraction. The results of three independent pairwise ChIP experiments (1, 2 and 3) are presented.