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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Gastroenterology. 2010 Feb 23;138(7):2531–2540.e4. doi: 10.1053/j.gastro.2010.02.043

Figure 4. Enhanced caerulein-induced damage in β1-Itgflox/flox;Ptf1a-Cre pancreata.

Figure 4

10 week old mice were injected hourly with 50 μg/kg caerulein for 7 hours and sacrificed 1 hour (A,B), 24 hours (C,D) and 1 week (E,F) after the final injection. Masson’s trichrome stained sections from β1-Itgflox/flox;Ptf1a-Cre pancreata with one hour recovery (B) indicated extensive vacuolization and loss of membrane integrity compared to wild type controls (A). Wild type pancreata recovered after 24 hours (C), while β1-Itg flox/flox;Ptf1a-Cre pancreata showed poor tissue integrity and persistence of inflammatory cells (D). 1 week after injection both wild type (E) and β1-Itg flox/flox;Ptf1a-Cre (F) pancreata had largely recovered but with aberrant acini commonly present in caerulein-treated β1-null pancreata. Arrows indicate centrally localized nuclei (F, inset). (Scale bar main picture= 40μm; inset= 80μm).