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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: J Mol Cell Cardiol. 2010 Feb 6;49(1):41–47. doi: 10.1016/j.yjmcc.2010.02.001

Figure 8.

Figure 8

A, MTT reduction assay in isolated rat cardiomyocytes. Cells were treated with NECA (100 nM) or ZnCl2 (5 μM) in the presence of pyrithione for 10 min. Both NECA (n = 6) and ZnCl2 (n = 6) reduced MTT reduction, indicating that zinc may mediate the cardioprotective effect of NECA by inhibiting mitochondrial metabolic activity. * p < 0.05 vs. control. B, Mitochondrial complex I activity assay in isolated rat hearts subjected to 30 min regional ischemia followed by reperfusion. NECA (100 nM, n = 4) and TPEN (10 μM, n = 4)) given 5 min prior to the onset of reperfusion for 35 min. Myocardial samples were collected 10 min the onset of reperfusion. NECA reduced the activity of the complex I and this was reversed by TPEN. * p < 0.05 vs. control; # p < 0.05 vs. NECA.