Skip to main content
. Author manuscript; available in PMC: 2010 Dec 10.
Published in final edited form as: Oncogene. 2010 Mar 29;29(23):3324–3334. doi: 10.1038/onc.2010.96

Figure 6. Phosphorylation dependencies that target Cdc25A to β-TrCP.

Figure 6

Cells transfected with plasmids expressing WT and mutant forms of Cdc25A for 20 h were lysed and lysates were resolved by SDS-PAGE. Western blotting was performed to monitor S76- (A), S88- (B), S82- (C) and S79 (D)-phosphorylation. (E) HeLa cells transfected with plasmids encoding tagged forms of Cdc25A and β-TrCP for 20 h were lysed and resolved directly by SDS-PAGE (WCE) or were incubated first with Flag agarose to precipitate Cdc25A. Precipitates were then resolved by SDS-PAGE followed by Western blotting. (F) Cells expressing WT and mutant forms of Cdc25A were either lysed immediately (time = 0) or were incubated in the presence of 10 μg/ml cycloheximide (CHX) for the indicated times prior to processing. Lysates were resolved by SDS-PAGE and subjected to Western blotting.