Table 1.
PEDF and VEGF production in human cardiac cells under normoxic and anoxic conditions
| HACM | PEDF | VEGF | ||
|---|---|---|---|---|
| Control | Anoxia | Control | Anoxia | |
| Donor 1 | 19.240 ± 0.413 | 9.511 ± 0.997* | 3.707 ± 0.939 | 7.193 ± 0.448* |
| Donor 2 | 18.056 ± 1.427 | 10.160 ± 1.131* | 3.215 ± 0.079 | 8.129 ± 0.716* |
| Donor 3 | 16.700 ± 1.124 | 8.350 ± 0.220* | 3.257 ± 0.252 | 10.969 ± 0.845* |
| Donor 4 | 15.468 ± 0.209 | 8.315 ± 0.243* | 3.824± 0.633 | 21.970 ± 0.367* |
| HACF | PEDF | VEGF | ||
| Control | Anoxia | Control | Anoxia | |
| Donor 1 | 10.235 ± 1.129 | 6.209 ± 0.217* | 2.876 ± 0.884 | 6.706 ± 0.812* |
| Donor 2 | 5.712 ± 0.252 | 2.655 ± 0.156* | 4.549 ± 0.338 | 12.560 ± 0.326* |
| Donor 3 | 7.806 ± 0.105 | 4.196 ± 0.123* | 3.181 ± 0.559 | 14.138 ± 1.051* |
| Donor 4 | 7.432 ± 0.856 | 3.783 ± 0.674* | 2.661 ± 0.619 | 7.772 ± 0.710* |
HACM and HACF isolated from explanted hearts from patients suffering from ischemic (donors 1and 2) and dilatative cardiomyopathy (donors 3 and 4) were cultivated under normoxic (control) and anoxic conditions for 48 hrs; PEDF and VEGF in the supernatant was determined by specific ELISA, values are given in ng/100,000 cells. Values represent mean values ± S.D. of three independent determinations. *P < 0.005.