Figure 4. MAA expression of ABCB5+ MMICs compared to ABCB5− tumor bulk populations.
(A) Representative dual-color flow cytometry analysis of malignant melanoma cells (MMC) costained for ABCB5 expression (APC, Fl4 fluorescence) and the MAAs ML-IAP, NY-ESO-1, MAGE-A, and (B) MART-1 (FITC, Fl1 fluorescence) in clinical patient-derived melanoma cells (left panel) and established G3361 melanoma cells (right panel). (C) MAA expression by ABCB5+ versus ABCB5− melanoma subpopulations (mean±SEM), as determined by dual-color flow cytometry (*: P<0.05; **: P<0.01). (D) Relative mRNA expression of TGF-β pathway members in ABCB5+ versus ABCB5− melanoma cells.