Skip to main content
. Author manuscript; available in PMC: 2011 Jan 15.
Published in final edited form as: Cancer Res. 2010 Jan 12;70(2):697–708. doi: 10.1158/0008-5472.CAN-09-1592

Figure 5. Effects of human melanoma cells on T cell activation and cytokine secretion.

Figure 5

(A) Left panel: 3H-thymidine uptake (mean cpm±SEM) of human PBMCs cultured in the presence or absence of PHA with or without addition of human melanoma cells (representative of n=4 independent experiments). Right panel: IL-2, (B) IL-10 (C) IFN-γ-, and (D) IL-5- (left), and IL-4 (right) production by PHA-stimulated PBMCs cultured in the presence or absence of melanoma cells as determined by ELISPOT analysis. Illustrated are mean spots per well±SEM of replicate wells representative of n=3-4 independent experiments (*, P<0.05, NS, not significant). Representative ELISPOT wells are shown.