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Mucus was markedly reduced following IN but not IP CpG treatment in TLR9+/+ and TLR9–/– mice. Mouse whole-lung tissue sections from Aspergillus-sensitized and challenged TLR9+/+ and TLR9–/–mice were stained with PAS. Representative whole lung sections from TLR9+/+ (a–c) and TLR9–/– mice (d–f) that were given no treatment (a, d), IN CpG (b, e), or IP CpG (c, f) are shown. Mucus stains purple using the PAS staining procedure (original magnification: ×200).
