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. Author manuscript; available in PMC: 2010 Jun 11.
Published in final edited form as: Autophagy. 2010 Jan 13;6(1):61–66. doi: 10.4161/auto.6.1.10326

Figure 1.

Figure 1

Induction of autophagy by fluspirilene. (A) Dose-dependent induction of LC3II by fluspirilene in H4 cells. H4 cells were treated with indicated concentrations of fluspirilene for 4 hours. The cell lysates were harvested and analyzed by western blotting using anti-LC3. Anti-tubulin was used as a loading control. (B) H4 cells were treated with fluspirilene (10 μM), bafilomycin A1 (100 nM), or fluspirilene and bafilomycin A1 together for 4 hours. 0.1% DMSO is used as negative control. The cell lysates were harvested and analyzed by western blotting using anti-LC3 antibody. Anti-tubulin was used as a loading control.