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. Author manuscript; available in PMC: 2010 Jun 11.
Published in final edited form as: Autophagy. 2010 Jan 13;6(1):61–66. doi: 10.4161/auto.6.1.10326

Figure 2.

Figure 2

The effects of fluspirilene on the levels of ATG12-ATG5 in H4 cells. (A) H4 cells were treated with 10μM fluspirilene for indicated length of time. The cell lysates were harvested and analyzed by western blotting using anti-ATG12 antibody. Anti-tubulin was used as a loading control. (B) H4 cells were treated with 10μM fluspirilene for indicated length of time. The mRNA levels of ATG5 are analyzed by RT-PCR as described in the methods. (C) H4 cells were treated with 10μM fluspirilene for indicated length of time. The cell lysates were harvested and analyzed by western blotting with anti-ATG5 antibodies. Anti-tubulin was used as a loading control.