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. Author manuscript; available in PMC: 2010 Oct 30.
Published in final edited form as: Cell. 2010 Apr 30;141(3):536–548. doi: 10.1016/j.cell.2010.02.025

Figure 5. Coupled MARCM for Clonal Analysis of Mutant Phenotypes.

Figure 5

(A) Schematic for coupled MARCM labeling of dividing cells during imaginal disc development.

(B) A control coupled MARCM clone. Both GAL4- and QF-labeled siblings are wild type. Genotype: hsFLP, QUAS-mtdT-HA, UAS-mCD8-GFP (X); ET40-QF, QUAS-mtdT-HA/+ (II); tubP-GAL4, 82BFRT, tubP-GAL80/82BFRT,tubP-QS (III).

(C) A coupled MARCM clone where GAL4-labeled sibling (green) is wild type, while QF-labeled sibling (red) is homozygous mutant for Tsc1. Genotype: hsFLP, QUAS-mtdT-HA, UAS-mCD8-GFP (X); ET40-QF, QUAS-mtdT-HA/+ (II); tubP-GAL4, 82BFRT, tubP-GAL80, Tsc1Q600×/82BFRT, tubP-QS (III).

Green, anti-CD8; Red, anti-HA; Blue, anti-fibrillarin (labels nucleoli). Scale bars: 20 μm.

(D-F) Quantification of clone area, cell number and cell size for experiments in B and C. n=30 for WT vs. WT; n=21 for WT vs. Tsc1. Error bars are ± SEM. ***, p<0.001.

Figure S5 shows additional characterization of the effects of QF, GAL4, or QF+GAL4 expression on imaginal disc differentation.