Table 3.
Linearity, Detection Limits , Accuracies and Variability for the Quantification of CBR1 and CBR3 (n=4)
|
aDL (fmol) |
aLinear range (fmol)/r2 |
bAccuracy% (RSD%) |
cRecovery% (RSD%) |
dMean accuracy% (RSD%) |
dMean recovery% (RSD%) |
|
|---|---|---|---|---|---|---|
| CBR1 | ||||||
| T1-2 | 0.08 | 2-600 /0.996 | 88(11); 93(7); 87 (7) |
92(6); 94(7) | 89(7); 93(5); 92(5) |
89(4); 96(9) |
| T1-5 | 0.015 | 2-600/0.990 | 91(4); 93(3); 96(5) |
86(6);97(10) | ||
| CBR3 | ||||||
| T3-2 | 0.11 | 0.2-60/0.992 | 97(9); 91(3); 94(4) |
92(5); 88(4) | 92(7); 93(4); 94(5) |
93(6); 90(3) |
| T3-4 | 0.09 | 0.2-60/0.986 | 87(5); 96(7); 95(8) |
95(11); 91(5) |
Detection limits were measured with the most sensitive peptide and are expressed as the protein amounts on column (assuming 100% efficiency for sample preparation).
Accuracies were measured by spiking the standard proteins at three levels (5, 50 and 250 fmol on-column for CBR1 and 1, 10 and 40 fmol for CBR3) into the blank matrix (rat liver cytosol), and then analyzing the samples twice in each of two successive days (n=4).
Recoveries were determined by spiking the standard proteins at two levels (20 and 200 fmol on-column for CBR1 and 4 and 30 fmol for CBR3) into a pooled human liver cytosol sample, and then measuring the samples twice in each of two successive days (n=4);
Accuracy and recovery calculated based on the averages of protein concentrations obtained independently by the two signature peptides.