Table 1.

K_ATP channel subunits-associated chaperone proteins^a

Accession no. (NCBI)	Protein name	Sequence coverage (%)	Unique spectra	Total spectra
fSUR1 only
Q09427	SUR1	46	143	588
Q61743	Kir6.2	11	4	4
Q76G10	Hsp40	26	7	9
P19378	Hsc70	57	43	72
P46633	Hsp90α	29	20	26
P11499	Hsp90β	39	30	43
O54981	HOP	16	6	6
Q8K3H8	Calnexin	13	7	10
fSUR1 + Kir6.2
Q09427	SUR1	48	126	377
Q61743	Kir6.2	21	10	18
Q76G10	Hsp40	37	9	12
P19378	Hsc70	61	33	57
P46633	Hsp90α	26	19	25
P11499	Hsp90β	34	24	35
O54981	HOP	7	3	3
Q8K3H8	Calnexin	13	7	8
HA-Kir6.2 only
Q09427	SUR1	8	7	9
Q61743	Kir6.2	30	22	100
Q76G10	Hsp40	29	6	10
P19378	Hsc70	57	42	95
P46633	Hsp90α	17	9	13
P11499	Hsp90β	23	13	18
O54981	HOP	0	0	0
Q8K3H8	Calnexin	0	0	0

^a In total, 10 proteomics experiments were performed: three in INS-1 cells infected with fSUR1 virus only, five in INS-1 cells infected with fSUR1 and Kir6.2 viruses, and two in INS-1 cells infected with HA-Kir6.2 virus only. The fSUR1 or HA-Kir6.2 protein complexes in INS-1 cell lysates were affinity purified using FLAG- or HA-antibody–conjugated agarose beads and subjected to mass spectrometry analyses as described in Materials and Methods. Results from three proteomic experiments representing each condition are shown. Note Hsp40, Hsc70, and Hsp90α and -β are present in all experiments. The protein HOP is found in only a subset of experiments overexpressing fSUR1 alone or fSUR1 plus Kir6.2 (6 of 8 experiments), so is the protein calnexin (5 of 8 experiments); neither proteins showed up in cells overexpressing HA-Kir6.2 only. NCBI, National Center for Biotechnology Information.