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The estimated properties of the synthetic luxR mRNA sequences. The properties of the synthetic RDS sequences, including their translational efficiencies (A), probabilities of ribosome-bound mRNA (B), overall RDS-exposure probabilities (C), and RRS-hybridization energies (D), were estimated and compared with normalized luxR expression levels averaged from three independent β-galactosidase assays. The translational efficiencies of the designed sequences were normalized for comparison.
