Figure 2.
In vivo multiplication, sFL production and antibody response to rVACV. Groups (n = 4) of C57Bl/6 female mice were inoculated i.p. with 1 × 106 pfu of P13-E/L-FL, P13-H5-FL or P13-E7. The ovaries and sera were collected at various intervals after infection. Replication of rVACV in the ovaries was determined by plaque assay (A) or by Q-PCR (B). The in vivo production of FL was measured in mouse sera by ELISA (C). To determine the immunogenicity of recombinants, groups of mice (n = 5) were i.p. inoculated with 1 × 106 pfu of rVACV. Five weeks later, the animals were anesthesized and the serum was collected. The levels of VACV specific IgG1 or IgG2a antibodies were quantified by ELISA (D). The data were analyzed by the two-way ANOVA.