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. 2010 Jun 11;5(6):e11025. doi: 10.1371/journal.pone.0011025

Figure 5. CDC27 but not USP22 or PI4KCA is required for modulation of phagosome biogenesis.

Figure 5

A. Representative confocal microscopy images that show co-localization of the late endosomal/lysosomal marker LAMP-2 with the phagosomes harboring the iglC mutant in the untreated HEK293T cells (UN) as well as the WT strain in the RNAi-treated cells at 2 h after infection. B. Representative confocal microscopy images that show co-localization of the lysosomal enzyme cathepsin D with the phagosomes harboring the iglC mutant in the untreated cells (UN), as well as the WT strain in the RNAi-treated cells. C. Quantification of co-localization of the phagosomes with LAMP-2 (L-2) and cathepsin-D (C–D) markers at various time points. The results are based on examination of at least 100 bacteria at 2 h after infection analyzed by confocal microscopy in 2 independent experiments performed in triplicate and the error bars represent standard deviation.