Figure 2. Kinetic analysis of PFV RNA and DNA synthesis.

A representative experiment is shown. U373-MG cells were infected in the presence or absence of AZT (100 µM). The drug was added 2 hours before infection and kept on cells during the experiment. Levels of intracellular PFV DNA and RNA were monitored during the first hours of infection. (A) Dynamics of viral RNA with and without AZT monitored by real-time RT-PCR. Synthesis and fate of total viral DNA (B), integrated DNA (C) monitored by real-time PCR. Viral RNA involved in the reverse transcription step as well as viral DNA synthesis were monitored (D). At each time point post-infection, quantification of viral RNA copies without AZT is subtracted from viral RNA copies with AZT.