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. Author manuscript; available in PMC: 2011 May 28.
Published in final edited form as: Chem Biol. 2010 May 28;17(5):471–482. doi: 10.1016/j.chembiol.2010.03.006

Figure 6.

Figure 6

(A) C646 inhibits WM983A melanoma cell proliferation but C37 does not. Cells were treated with each compound for 24 h. Proliferation was measured via 3H-thymidine incorporation. (B) C646 blocks H3 acetylation in WM983A cells. Cells were treated for 6 h with increasing concentrations of C646. Nuclear lysates were subjected to western blot analysis for acetylated H3 (Upstate 06-599). Total H3 (Abcam ab1791) was blotted as a loading control. (C) C646 causes growth arrest in melanoma cell line WM35 and WM983A, indicated by a decrease in %S phase. Cells were treated for 24 h with 20 μM C646 or DMSO, then collected and stained with propidium iodide, followed by FACS analysis.