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. Author manuscript; available in PMC: 2011 Mar 16.
Published in final edited form as: Biochemistry. 2010 Mar 16;49(10):2246–2255. doi: 10.1021/bi902066t

Figure 4.

Figure 4

pH-dependence of LpxC-catalyzed deacetylase activity for Zn2+-C63A (●) or Fe2+ - C63A (○) LpxC. The values of V/K were measured at 30 °C using subsaturating concentrations of myr-UDPGlcNAc (≤ 0.2 μM) and enzyme reconstituted with stoichiometric metal ion, as described under “Materials and Methods”. The pKa values (see Table 1) were determined by fitting Eq. 1 to the data.