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Dependence of LpxC activity on reducing agents. (A) The activity of Fe2+- and Zn2+-EcLpxC was measured at 30 °C in buffer containing either 10 mM (black bars) or 0.5 mM (gray bars) TCEP as described in the “Materials and Methods” section. (B) Apo-EcLpxC was reconstituted with either Fe2+ (open circle) or Zn2+ (filled circle) and the resulting activity was measured at 30 °C (20 mM bis-tris propane, 10 mM TCEP, pH 7.5) as a function of time.
