Table 2. TN5EZ insertions that reduced S. aureus hemolysin activity.
| # of mutants* | Hemolysis | Gene/Locus | Function or predicted function |
| 1 | LH | SA0466 | Membrane protein, putative |
| 1 | LH | bioD | Dethiobiotin synthase - Biosynthesis of cofactors |
| 2 | LH | SA0555 | Peptidase, M41 - Degradation of proteins, peptides, |
| 1 | LH | SA0635 | Lipoate-protein ligase A - Protein modification |
| 1 | LH | SA0762 | Hemolysin, putative - |
| 3 | LH | gcvH | Glycine cleavage system H protein - energy metabolism |
| 1 | LH | SA0958 | General stress protein 13 |
| 1 | LH | SA1049 | 1,4-dihydroxy-2-naphthoate octaprenyltransferase, putative |
| 1 | LH | SA1220 | Fibronectin-binding protein A-related |
| 2 | LH | SA1294 | Metallo-beta-lactamase superfamily protein |
| 1 | LH | glpD | Aerobic glycerol-3-phosphate dehydrogenase |
| 1 | LH | SA1563 | 2-oxoisovalerate dehydrogenase, lipoamide dehydrogenase, |
| 1 | LH | secDF | Protein-secretion, membrane protein, SecDF family |
| 1 | LH | SA1751 | DHH family protein, putative |
| 2 | LH | ackA | Acetate kinase - fermentation |
| 1 | LH | ccpA | Catabolite control protein - DNA interactions |
| 1 | LH | agrA | Accessory gene regulator protein A |
| 1 | LH | SA2027 | Hypothetical protein - not conserved |
| 9 | LH | atpDGA | ATP synthase F1, beta, gamma and alpha subunits - |
| 1 | LH | SA2271 | Molybdenum ABC transporter, permease protein - cations |
| 1 | LH | SA2375 | Transporter - unknown substrate |
| 1 | LH | SA2534 | NAD(P)H-flavin oxidoreductase |
| 1 | LH | SA2572 | Cation-transporting atpase, E1-E2 family |
| 1 | LH | SA2624 | Acetyl-CoA synthetase, putative |
| 3 | LH | SA2735 | Chromosome partioning protein, ParB family |
*LH represents lower hemolysis compared to WT and most other Tn5EZ mutants on sheep blood agar (also see Fig. 1).