Table 1. Immunization with HIVBr18 induces CD4+ T cell proliferation in human HLA- class II transgenic mice.

Stimulus	DR2	DR4	DQ6	DQ8
p17(73–89)		0.86	1.72
p24(33–45)
p24 (131–150)
p6(32–46)			1.57
pol (63–77)		0.40	1.64
pol (136–150)		0.13	3.27
pol (785–799)			2.54
gp41(261–276)
gp160(19–31)		0.35	1.42
gp160(174–185)			1.35
gp160(188–201)		0.31	4.33
gp160(481–498)
rev (11–27)			1.42
vpr (58–72)
vpr (65–82)
vif (144–158)			3.24
vpu (6–20)
nef (180–194)	14.17	2.00	1.46
cutoff	6.89	0.10	1.21	2.40
recognized peptides	1	6	11	0

Quantitative analysis of proliferating CD4⁺ T cells (gated on CD3⁺CD4⁺ CFSE^low cells) from HIVBr18 immunized transgenic mice. Proliferative responses measured 15 days after immunization. Only proliferative (%CFSE^low) responses above cutoff are shown. Cutoff values for each transgenic strain are shown in the table. % CFSE^low cell values were calculated after subtraction of % CFSE^low cells in unstimulated cultures. Since we observed different background values in the CFSE assay among each mouse strain, individual cutoffs were established to distinguish the random noise from the true proliferation values. The nonspecific proliferative response was found to be from 0.10% to 6.89%.