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(a) Fluorescence spectra of (1) ATMND, (2) ATMND/17Sva/17Eva, (3) ATMND/17Sva/17Eva/Pb2+, (4) ATMND/17Sva/17Eva/Pb2+/EDTA. (b) Kinetics of fluorescence enhancement of ATMND/17Sva/17Eva in the presence of different amounts of Pb2+. Condition: 25 mM HEPES pH 7.0 and 100 mM NaCl at 5 °C. λex/λem = 358/405 nm.
