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(A) Gel electrophoresis results of anti-CEA antibody reduction at TCEP (reducing agent) concentration of 0×, 3×, 10× and 30× over the concentration of the antibody, respectively. The rightmost lane represents a standard molecular weight ladder. (B) Quantifying the conjugation yield of 1 mg of maleimide-terminated hybrid NPs with 50 μg of anti-CEA antibody reduced by 3× TCEP versus 50 μg of untreated anti-CEA antibody.
