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. Author manuscript; available in PMC: 2011 Jun 11.
Published in final edited form as: Circ Res. 2010 Apr 8;106(11):1753–1762. doi: 10.1161/CIRCRESAHA.109.196030

Figure 4.

Figure 4

Figure 4

Figure 4

Silencing of endogenous CCL7 ablated the protective effects of CCR1-overexpression. (A) Effect of siRNA on CCL5 and CCL7 mRNA expression in MSCs. RT-PCR for CCL5, CCL7 and β-actin at 72h after siRNA transfection. (B) Real-time RT-PCR for relative CCL5, CCL7 mRNA levels of MSCs after siRNA transfection. Changes are expressed as percentage of remaining mRNA versus control siRNA treated MSCs. (C) Quantitative analysis of apoptosis in percentage of TUNEL positive control MSCs or CCR1-MSCs transfected with siRNAs and then subjected to serum deprivation for 12h. Percentages of TUNEL positive control MSCs or CCR1-MSCs are shown and the data are expressed as mean ± SD. n = 3–4; *P < 0.05 compared with scrambled siRNA treated CCR1-MSCs; #P < 0.05 versus control MSCs; N.S.: no significance.