(A) Same as Fig. 4A, except that CsA (2.5 μM) was added to GST, GST-CypA and GST-H126Q CypA 15 min prior to the addition of NS5A Con1-His. (B) Same as A, except that increasing concentrations of CsA were used: 0 (lane 2), 0.3125 (lane 3), 0.625 (lane 4), 1.25 (lane 5) and 2.5 μM (lane 6). (C) Same as 1B. (D) Huh7 cells were co-transfected with pBIND, pact and pG5luc. Three days post-transfection, luciferase activity was measured in cell lysates. Data (triplicates) are representative of four independent experiments.