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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: J Hepatol. 2010 Apr 3;53(1):50–56. doi: 10.1016/j.jhep.2010.01.041

Fig. 4. The D320E NS5A Mutation Does Not Render CypA-NS5A Interactions Resistant to Cs.

Fig. 4

(A) GST-CypA (100 ng) preincubated for 15 min with increasing concentrations of CsA (from 0.3125 to 1.25 μM) was mixed with recombinant wild-type or D320E mutant NS5A-His (10 ng) for 3 h at 4 C. Glutathione beads were added to the GST-CypA/NS5A mixture for 30 min at 4° C and washed. Bound material was eluted and analyzed by Western blotting using anti-His antibodies. (B) Same as 2C.