Figure 5.
Expression of TGF-β1 and IFN-γ in injured and control tibialis anterior (TA) muscle homogenates. (a) Representative samples at 8 h postinjury quantified by Western blot analysis for TGF-β1. Samples were all from the same gel, but cut with Adobe Photoshop for appropriate presentation. Photoshop editing was performed to all samples equally. (b) Relative TGF-β1 levels in ischaemia–reperfused (I-R) and contused muscle at 8 h and 3 days postinjury were determined by densitometry of Western blots and averages from each group are displayed in centre panel. (c) IFN-γ concentration (pg/mg total protein) was quantified by ELISA in TA muscles after I-R, contusion and I-R combined with contusion and compared to uninjured controls. * denotes a significant difference from control, P<0.05. ø denotes a significant difference from earlier time point within the same experimental group, P<0.05. ψ denotes a significant difference between contusion and IR-contusion at same time point, P<0.05. Error bars represent the SE of the mean (SEM, n=8 per group).