Figure 12.

Coomassie brilliant blue-stained SDS-PAGE gel. Electrophoretic fractionation of simultaneous overexpression of PgHsp70 along with CA prevents the aggregation and accordingly prevents the formation of inclusion bodies in E. coli: Lane 1 is molecular weight of marker lane; the sizes (in kDa) are indicated adjacent (left side) to the gel. Lane 2 is co-expression of CA with PgHsp70 showing the high level of overproduction of CA in supernatant after after 3 h induction with IPTG (IPTG induced supernatant fraction). Lane 3 is induced CA in the pellet fraction (without PgHsp70); i.e., without co-expression the CA protein was going to pellet fraction. Lane 4 is a pellet fraction of only PgHsp70. Lane 5 is pellet fraction after co-expression of CA with PgHsp70; the CA and PgHsp70 proteins are not appearing. Lanes 4 and 5 were run in another gel and Coomassie Brilliant Blue stained.