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Effect of anion charger KI: (A) on chaperone activity of native PgHsp70 in 0.1 M phosphate buffer (pH 8.0) at 50°C for 5 min. (B) on secondary structure of PgHsp70 [spectra 1 & 2–5 represents native PgHsp70, different concentration of KI (0.2–0.5 mM) with native PgHsp70] (C) Effect of acrylamide on chaperone activity experimental conditions as mention in Figure 5. The error bars have not been added in this figure because the SD in all the cases was less than 10%.
