Table 2.

Effect of different physical (temperature and pH) and chemical agents (denaturants and inhibitors) on secondary structure of PgHsp70

Effect	α-helix	3/10 helix	Parallel β-sheet	Antiparallel β-sheet	Beta turn	Unordered	Total
Control (PgHsp 70)	0.582	0.222	−0.001	0.000	0.051	0.144	0.996
Temp. 35°C–85°C	0.582	0.222	−0.001	0.000	0.051	0.144	0.996
pH (4, 8 and 10)	0.582	0.222	−0.001	0.000	0.051	0.144	0.996
PgHsp 70 + Gdn.HC l (0.1 mM)	0.069	0.138	0.172	0.069	0.190	0.363	1.000
PgHsp 70 + Gdn.HC l (0.1 mM) + glycerol (0.001%)	0.582	0.222	−0.001	0.000	0.051	0.144	0.996
PgHsp 70 + KI (0.1 mM)	0.006	0.086	0.300	0.161	0.110	0.340	1.000
PgHsp 70 + acrylamide (0.1 mM)	0.542	0.262	−0.001	0.000	0.051	0.144	0.996
PgHsp 70 + Urea (0.1 mM)	0.059	0.238	0.223	0.223	0.044	0.214	1.000