. 2010 Feb;5(2):136–139. doi: 10.4161/psb.5.2.10369

Table 1.

Monosaccharide compositional analysis of the wall fractions derived from culms of mutant and wild-type plants

Fractions		RHA	FUC	ARA	XYL	MAN	GLC	GAL	Total
EPG	WT	0.2	0.1	0.3	0.9	1.0	0.6	0.2	3.3
EPG	oscsld4	0.3	0.3	0.6	1.2	0.9	0.6	0.5	4.5
Na₂CO₃	WT	0.1	0.4	0.2	0.4	0.1	0.3	0.2	1.6
Na₂CO₃	oscsld4	0.1	0.2	0.4	0.6	0.1	0.7	0.3	2.4
1 N KOH	WT	0.2	0.1	6.1	34.7	0.1	2.8	1.4	45.4
1 N KOH	oscsld4	0.2	0.1	5.8	23.4	0.1	5.4	2.0	37.0
4 N KOH	WT	0.1	0.1	1.1	8.5	0.1	3.0	0.4	13.2
4 N KOH	oscsld4	0.1	0.1	1.3	5.7	0.1	6.4	0.8	14.6
4 N insol	WT				294.7
4 N insol	oscsld4				252.6
Total	WT	0.5	0.7	7.8	44.5	1.2	301.4	2.2	358.2
Total	oscsld4	0.7	0.8	8.2	30.9	1.2	265.7	3.7	311.2

The alcohol insoluble wall residues (AIR) were extracted from the second internodes of oscsld4 and wild-type culms; The destarched AIR was fractioned sequentially with endopolygalacturonase (EPG) plus pectin methylesterase (PME), 50 mM Na₂CO₃, 1 N KOH, and 4 N KOH; The fractions were hydrolyzed with sulfuric acid and the alditol acetates were analyzed by GC-MS; The insoluble residue after 4 N KOH extraction was used to determine cellulose content by colorimetric assay; The results are given as means (mg g⁻¹ of AIR) of four independent assays; The variance was not shown but is <15%.