Skip to main content
. Author manuscript; available in PMC: 2010 Jun 12.
Published in final edited form as: J Immunol. 2008 Sep 15;181(6):3811–3817. doi: 10.4049/jimmunol.181.6.3811

FIGURE 1.

FIGURE 1

pDC and myDC express FcγR (CD16/CD32) and take up IC in vivo. a and b, Expression of FcγR (CD16/CD32) on DC subsets isolated from spleen (a) or lymph nodes (b). c, IC containing fluorescent DQ-OVA was injected into the left footpads of mice and, after 8 h, draining popliteal and superficial inguinal lymph nodes were isolated. Contralateral (nondraining) popliteal lymph nodes served as control. d, Uptake of IC OVA-IC at 5 h postinjection. e, Uptake of DQ-OVA 8 h postinjection. IC or DQ-OVA uptake was analyzed by four-color flow cytometry. Gates were set on CD11c+ DC, excluding cells in the PE channel, dead cells, and debris. IC uptake was measured in the FITC channel, and pDC or myDC were detected using PE-Cy5-conjugated mAb to B220 and CD11b, respectively. Data are representative of three independent experiments.