FIGURE 6.

pDC can cross-present IC-derived Ag to CD8⁺ T cells in vivo. FACS-sorted DC subsets (1–2 × 10⁶) incubated with OVA-IC overnight were injected in the footpad, and CFSE-labeled, CD8⁺ OVA-Tg T cells (8–10 × 10⁶) were injected i.v. in the base of the tail. a, After 2 days, draining lymph nodes were harvested and CD8⁺ (OT-I) T cell activation was determined. b, Lymph node T cells were analyzed for their production of cytokines after restimulation with PMA and ionomycin. OVA-Tg CD8⁺ T cells were gated based on forward and side scatter and TCR Vα2 expression, and IFN-γ- and IL-10-producing cells were determined. *, Indicates p < 0.05. Data shown are representative of five independent experiments.