Table 1.
Candidate reference and target genes.
| Candidate Reference Genes | |||||
|---|---|---|---|---|---|
| Symbol | Name | Function | Assay ID& | r2 | E |
| ACTB | Actin, beta | Structural protein | Hs99999903_m1 | 0.997 | 1.97 |
| B2M | Beta-2-microglobulin | Beta chain of MHC I | Hs99999907_m1 | 0.999 | 2.00 |
| CTBP1 | C-terminal binding protein 1 | Involved in cellular proliferation. | Hs00179922_m1 | 0.998 | 2.09 |
| GAPDH | Glyceraldehydes-3-phosphate dehydrogenase | Glycolysis and gluconeogenesis | Hs99999905_m1 | 0.998 | 2.00 |
| GOLGA1 | Golgi autoantigen, golgin subfamiliy A, 1 | Unknown function | Hs00608118_m1 | 1.000 | 1.96 |
| PGK1 | Phosphoglycerate kinase 1 | Glycolysis | Hs99999906_m1 | 0.997 | 2.07 |
| PPIA | Cyclophilin A (Peptidylprolyl isomerase A) |
Protein folding | Hs99999904_m1 | 0.996 | 2.08 |
| PPIB | Cyclophilin B (Peptidylprolyl isomerase B) |
Associated with the secretory pathway | Hs00168719_m1 | 0.996 | 2.00 |
| SDHA | Succinate dehydrogenase complex, subunit A | Electron transporter | Hs00417200_m1 | 0.998 | 2.10 |
| TMBIM4 | Transmembrane BAX inhibitor motif containing 4 | Involved in apoptosis | Hs00211390_m1 | 1.000 | 2.00 |
| TBP | TATA box binding protein | Transcription factor | Hs99999910_m1 | 0.999 | 2.10 |
| UBC | Ubiquitin C | Protein degradation | Hs00824723_m1 | 0.999 | 2.05 |
| Target Genes | |||||
| Symbol | Name | Function | Assay ID | r2 | Efficiency* |
| IL10 | Interleukin 10 | Antiinflammatory cytokine | Hs00174086_m1 | 0.989 | 2.01 |
| TNF-α | Tumor necrosis factor | Proinflammatory cytokine | Hs00174128_m1 | 0.997 | 2.08 |
r2, Square of Pearson's correlation coefficient. E, Efficiency as calculated from 10-fold serial dilutions. &Assay IDs from Applied Biosystems