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Real-time kinetic measurement of PSA protease activity. (a) SERS spectra for 6 nM PSA incubation taken over 30 minutes with an integration time of 30 seconds. (b) SERS spectra for the negative control, 6 nM granzyme B, taken over 30 minutes. (c) Time-resolved measurements of relative change in Raman peak intensity at 1316 cm-1, 1456 cm-1, 1526 cm-1, and 1597 cm-1 before and after addition of PSA protease. Negative time represents time before protease addition.
