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Diffusion time (τD) distributions of TMR-labeled pHLIP bound to a supported lipid bilayer of POPC at pH 8 and different total peptide concentrations: (A) 1 nM and (B) 1 μM. (Inset) Diffusion time (τD) distribution of Texas Red-DHPE (0.002 mol %) in supported lipid bilayer of POPC at pH 8.
