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COS-7 cell transiently transfected with Httex1p-25QP-EGFP. Cells were imaged 51 h after transfection with 0.8% laser power at 488 nm and 20 μs/pixel. (A) Intensity image of a single plane shows widespread presence of Httex1p-25QP-EGFP, slightly lower levels in the nucleus. (B) Selection of the pixels with brightness corresponding to the monomers (B = 1.074, ɛ = 3700 cpsm). (C) Pixel brightness histogram confirms the prevalence of monomers.
