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Kinetics of hLFABP-ANS interaction. The hLFABP-ANS samples were prepared by adding ANS into the solution of hLFABP with final molar ratios (ANS/hLFABP) of (A) ∼1:4 and (B) ∼1:2, respectively. The experimental data of representative residues recorded at 500 MHz (o) and 800 MHz (∗) fields were fitted with the two-state exchange model (solid lines) globally as described in the Materials and Methods.
