Fig. 5. Mitochondrial complex I expression and activity in A-CREB and WT mice following SE.

(a) Left) Western blotting for ND6, a subunit of complex I. In wild type mice, SE induced a modest increase in ND6 expression at 3 days post-SE. In A-CREB transgenic mice, SE did not elevate ND6 levels. In addition, relative to WT mice, a lower level of ND6 expression was detected in A-CREB transgenics. Blots were probed for the transgene marker (GFP) and for total protein levels (ERK 1/2). Two samples were run for each condition. Right) ND6 band intensity was normalized to ERK 2 intensity for each lane; control (no SE) values were normalized to a value of 1. (b) Representative triple labeling for GFP, DraQ5 and ND6 (complex I). The images reveal that transgenic cells have a lower level of ND6 expression than non-transgenic cells. Arrows denote GFP-positive cells. Arrowheads denote GFP-negative cells. (c) Complex I activity. Striatal mitochondria were isolated from A-CREB and WT mice under control conditions and three days after SE. Complex I activity (assayed in the crude mitochondrial preparation) was normalized to a value of 1 for WT mice. Values are presented as the mean ± SEM. The numbers in the bars denotes the number of animals (samples) used for the analysis.