Figure 6.
Sam68 correlates with cyclin D1b levels in human PCa. A, representative fluorescence-based immunohistochemistry for Sam68 (top), cyclin D1b (middle), and cyclin D1a (bottom) in serial sections from two human PCa specimens with low (Tumor 1) and high Sam68 (Tumor 2). Red fluorescence, Sam68, cyclin D1b, and cyclin D1a; green fluorescence, epithelial cytokeratin; blue fluorescence, 4′,6-diamidino-2-phenylindole–stained nuclei. B, correlation analysis between Sam68 and cyclin D1b (left) or cyclin D1a (right) using nuclear intensities obtained from cytokeratin-positive epithelia using the AQUA system. Linear regression analysis is indicated by an r2 value. Spearman correlation statistics are indicated by a P value.