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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Neurobiol Dis. 2009 Jul 24;36(2):259–268. doi: 10.1016/j.nbd.2009.07.014

Figure 1. 3-NP intoxication regulates striatal CREB phosphorylation.

Figure 1

Top: time course depicting the 3-NP injection and sacrifice schedule for experiments presented in A–C. A. Cresyl violet (CV) histology reveals marked 3-NP-induced cell damage within the lateral striatum. Tissue was collected from mice sacrificed 1 day after the last 3-NP injection. Uniform CV labeling was detected throughout the striatum of vehicle-infused tissue. Scale bar = 400 μm. B. Immunoreactivity for the Ser-133 phosphorylated form of CREB (pCREB). Top) Representative section from a vehicle-injected animal: note the modest level of pCREB expression. Boxed region (B1) is magnified to the right. Bottom). Marked pCREB expression was detected within the penumbral region surrounding the foci of 3-NP-evoked striatal damaged (magnified in B2). Limited pCREB expression was observed within the 3-NP ‘core’ (magnified in B3). Scale bars: 400 μm in the low magnification image and 100 μm in the enlarged image. C. Representative immunofluorescence image within the penumbral region shows that pCREB co-labels with NeuN, indicating CREB is activated in striatal neurons. Scale bar = 50 μm.