TABLE 1.
Commercial Transfection Reagents Tested in HUVEC
| Transfection reagent | Formulation | Reagent (μL):DNA (μg)/well (9.4 cm2) | Manufacturer |
|---|---|---|---|
| Effectene | Nonliposomal lipid | 20:2 | Qiagen GmbH (Hilden, Germany) |
| 8:0.8 | |||
| 20:0.8 | |||
| 40:0.8 | |||
| SuperFect | Activated dendrimer | 10:5 | Qiagen GmbH |
| 4:2 | |||
| 10:2 | |||
| 20:2 | |||
| Escort IV | Polycationic lipid and neutral nontransfecting lipid | 20:5 | Sigma-Aldrich (St. Louis, MO, USA) |
| 8:2 | |||
| 4:1 | |||
| ExGen 500 | Cationic polymer of linear 22 kDa PEI | 16.5:5 | Fermentas International Inc. (Burlington, Ontario, Canada) |
| 9.87:3 | |||
| 8.23:3 | |||
| 11.52:3 | |||
| FuGene 6 | Lipids and other components | 3:1 | Roche (Basel, Switzerland) |
| 3:2 | |||
| 6:1 | |||
| FuGene HD | Lipids and other components | 15:5 | Roche |
| 6:2 | |||
| GeneJammer | Polyamine and other components | 6:2 | Stratagene (La Jolla, CA, USA) |
| 9:3 | |||
| Lipofectamine 2000 | Cationic lipid | 2:4 | Invitrogen (Carlsbad, CA, USA) |
| 8:4 | |||
| 10:4 | |||
| 10:5 | |||
| Lipofectamine LTX | Cationic lipid | 7.5:2.5 | Invitrogen |
| 3.75:2.5 | |||
| 6.25:2.5 | |||
| 3:1 |