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View full-text article in PMC

. Author manuscript; available in PMC: 2010 Jun 14.

Published in final edited form as: Antivir Ther. 2008;13(7):851–862.

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Comparison of the antiviral effects rendered by the IFNs on HCV RNA replicon containing cells. (A) Immunoblot analysis of Huh7 cells (lanes 1-4 on both panels) and Huh7 cells harboring the HCV RNA replicons after incubation with the C_max of each IFN for the indicated times. HCV NS5A protein levels were quantified by densitometry analysis and the values are displayed below the corresponding lane as a percentage of the protein within matched control cells cultured with media alone. Control values from nontreated cells were set at 100%. (B) ELISA of NPT II protein levels from HCV-K2040 (green) and HCV-HP (red) IFN treated cells. The calculated EC₅₀ of each IFN is displayed under the corresponding graph. Data are representative of three independent experiments. (C) Immunoblot analysis of endogenous IPS-1, NS5A and actin expression in control Huh7 cells (lanes1-4), Huh7-L2198S cells (lanes 5-8) and Huh7-K2040 cells (lanes 9-12) cultured with media alone (0) or C_max of each IFN for 72 hours. Arrows denote the full length (FL) and cleaved forms of IPS-1.

Comparison of the antiviral effects rendered by the IFNs on HCV RNA replicon containing cells. (A) Immunoblot analysis of Huh7 cells (lanes 1-4 on both panels) and Huh7 cells harboring the HCV RNA replicons after incubation with the C_max of each IFN for the indicated times. HCV NS5A protein levels were quantified by densitometry analysis and the values are displayed below the corresponding lane as a percentage of the protein within matched control cells cultured with media alone. Control values from nontreated cells were set at 100%. (B) ELISA of NPT II protein levels from HCV-K2040 (green) and HCV-HP (red) IFN treated cells. The calculated EC₅₀ of each IFN is displayed under the corresponding graph. Data are representative of three independent experiments. (C) Immunoblot analysis of endogenous IPS-1, NS5A and actin expression in control Huh7 cells (lanes1-4), Huh7-L2198S cells (lanes 5-8) and Huh7-K2040 cells (lanes 9-12) cultured with media alone (0) or C_max of each IFN for 72 hours. Arrows denote the full length (FL) and cleaved forms of IPS-1.