Figure 7.
Sleep deprivation increases resistance to infection. A. Left panel: Representative bacterial cultures obtained from Canton- S flies infected with E. coli immediately following a 4h deprivation (DEP; lower), or from handled controls (HC; upper). Plates are a 1:10 dilution of the homogenate. Right panel: the average number of colony forming units (cfu) per fly is plotted for flies from several strains including CS, y w, and rosy (see text; * = P <0.05; n=9) B. Effect of deprivation is limited to a time of day when flies are predicted to be resting. y w flies were sleep deprived and infected at the times indicated. Cfu/fly is significantly greater in HC as compared to DEP at ZT8 (* = P <0.05; n=4). No significant difference is observed between HC and DEP at ZT2, when flies are predicted to be active.