. Author manuscript; available in PMC: 2011 May 15.

Published in final edited form as: Anal Chem. 2010 May 15;82(10):4078–4088. doi: 10.1021/ac1001383

Table 1.

¹H- and ¹⁵N-chemical shifts (ppm) of amide protons of ¹⁵N-acetylhexosamines from GAG types, unsaturated dimer derivatives, and commercial standards.

GAG types or derivatives	major amino sugar	figure	chemical shifts ^a (ppm)
			¹H	¹⁵N
CS-A	β-GalNAc-4(SO₃⁻)	3A	7.963	120.91
CS-C	β-GalNAc-6(SO₃⁻)	3B	7.942	121.61
OSCS	β-GalNAc-4,6di(SO₃⁻)	3C	7.941	121.21
DS (CS-B)	β-GalNAc-4(SO₃⁻)	3D	8.096	120.46
HS	α-GlcNAc-6(SO₃⁻)	3E	8.361	123.62
ΔC4S dimer	α-GalNAc-4(SO₃⁻)	4A	8.353	120.60
ΔC4S dimer	β-GalNAc-4(SO₃⁻)	4A	8.138	121.40
ΔC6S dimer	α-GalNAc-6(SO₃⁻)	4B	8.241	121.80
ΔC6S dimer	β-GalNAc-6(SO₃⁻)	4B	8.092	122.75
GlcNAc (standard)	α-GlcNAc	S1A	8.138	122.95
GlcNAc (standard)	β-GlcNAc	S1A	8.039	123.72
GalNAc (standard)	α-GalNAc	S1B	8.080	122.61
GalNAc (standard)	β-GalNAc	S1B	7.972	123.53

Chemical shifts are relative to trimethylsilylpropionic acid at 0 ppm for ¹H and ¹³C, and liquid ammonia for ¹⁵N, obtained in experiments at 25 °C and pH 4.5.