TABLE 2.
Conservation of cell division proteins in Acinetobacter spp.
| Protein | Function | Protein locationa | Protein length (amino acids)b | % Sequence identity (residue range) |
Essentialitye | Homologs with known structuresb (residue range of sequence match; % sequence identity) | Known protein-protein interactions in E. coli | Reference(s) | ||
|---|---|---|---|---|---|---|---|---|---|---|
| A. baylyi vs E. colic | A. baumannii vs E. colid | A. baylyi vs A. baumanniic | ||||||||
| FtsA | Tethers FtsZ to membrane, stabilizes Z rings at membrane | c | 420 | 29 (8-409) | 30 (8-409) | 88 (1-420) | A, E, P | Thermotoga maritima (10-351; 23) | FtsZ | 101, 207, 249, 257 |
| FtsB (DivIC) | Unknown; role in septal peptidoglycan synthesis? | m | 110 | 41 (6-77) | 38 (25-96) | 91 (6-110) | A, E, P | —f | FtsQ, FtsL | 28, 92 |
| FtsE | Unknown; similar to ABC transporter | c | — | — | — | — | E | — | FtsZ, FtsX | 46, 218, 227 |
| FtsI/PBP3 (PBPB) | Cross-links septal peptidoglycan | m | 621 | 40 (15-607) | 39 (7-598) | 89 (10-617) | A, E, P | — | FtsQ, FtsN | 66, 69 |
| FtsK | Role in chromosome segregation and septum formation | m | 1018 | 60 (528-1017) | 59 (516-1009) | 77 (1-1018) | A, E, P | Pseudomonas aeruginosa (527-942; 59), E. coli (528-1017; 59) | FtsZ, FtsL, FtsQ | 66, 169 |
| FtsL | Unknown; role in septal peptidoglycan synthesis? | m | 110 | 24 (26-100) | 26 (28-100) | 88 (29-109) | A, E, P | — | FtsQ, FtsB, FtsW, FtsK | 28, 66, 69 |
| FtsN | Unknown | m | 200 | 23 (44-197) | 16 (3-206) | 87 (1-200) | A, E, P | E. coli (120-200; 19) | FtsQ, FtsI, FtsW | 52, 53, 66, 69, 268 |
| FtsQ (DivIB) | Unknown; role in septal peptidoglycan synthesis? | m | 284 | 25 (61-261) | 26 (90-259) | 81 (1-284) | A, E, P | Yersinia enterocolitica (59-266; 24), E. coli (61-267; 23) | FtsB, FtsL, FtsI, FtsN, FtsW, FtsK | 28, 34, 69, 220, 250 |
| FtsW | Septal peptidoglycan synthesis | m | 399 | 42 (32-388) | 42 (31-387) | 85 (2-399) | A, E, P | — | FtsI, FtsL, FtsN, FtsQ | 66, 69 |
| FtsX | Unknown; similar to ABC transporter | m | — | — | — | — | E | — | FtsE | 218, 227 |
| FtsZ | First protein to form midcell ring in cell division | c | 389 | 47 (31-386) | 48 (31-388) | 90 (1-389) | A, E, P | P. aeruginosa (31-322; 55), B. subtilis (35-389; 46), Mycobacterium tuberculosis (31-322; 53), Methanococcus jannaschii (5-322; 42), A. aeolicus (31-320; 43) | FtsA, ZapA, ZapB, ZipA, FtsE, FtsK, MinC, SulA, ClpX, SlmA | 22, 46, 66, 71, 79, 95, 98, 101, 108, 110, 151, 160, 161, 165, 179, 193, 194, 207, 228, 257 |
| ZapA | Promotes FtsZ polymerization and protofilament bundling | c | 94 | 21 (7-94) | 26 (1-88) | 80 (1-92) | D | Pseudomonas aeruginosa (1-93; 16) | FtsZ | 95, 160 |
| ZapB | Promotes FtsZ polymerization | c | — | — | — | — | D | — | FtsZ | 71 |
| ZipA | Stabilizes Z rings at membrane | m | 325 | 28 (185-310) | 27 (205-330) | 62 (1-324) | E | E. coli (183-323; 26) | FtsZ | 98, 101, 179 |
| MinC | cell division inhibitor | c | 240 | 40 (111-230) | 41 (122-229) | 78 (1-240) | D | T. maritima (111-213; 28) | MinD, FtsZ | 47, 108-110, 193, 228 |
| MinD | Membrane ATPase of the MinC-MinD-MinE system | m | 278 | 64 (9-278) | 65 (9-278) | 95 (1-278) | E | Archaeglobus fulgidis (9-277; 30), Pyrococcus furiosus (9-256; 30) | MinC, MinE | 48, 104, 108-110, 224 |
| MinE | Cell division topological specificity factor | c | 90 | 33 (9-88) | 32 (9-88) | 92 (1-90) | E | E. coli (36-90; 28) | MinD | 62, 110, 147, 214, 278 |
| SlmA | Spatial regulation of Z-ring formation (nucleoid occlusion) | c | — | — | — | — | D | — | FtsZ | 22 |
| SulA | FtsZ assembly inhibitor | c | — | — | — | — | D | P. aeruginosa (no homolog) | FtsZ | 49, 110 |
| ClpX | FtsZ assembly inhibitor | m | 436 | 70 (10-408) | 71 (10-408) | 91 (1-436) | D | Helicobacter pylori (54-417; 58) | FtsZ | 32, 79, 146 |
| AmiC | Amidase; required for septal wall degradation and cell separation | p | — | — | — | — | D | — | — | 20 |
| EnvC | Murein hydrolase; required for septal wall degradation and cell separation | p | — | — | — | — | D | — | — | 21 |
a
c, cytoplasmic; p, periplasmic; m, membrane associated.
b
Acinetobacter baylyi protein (15).
e
A, essential in Acinetobacter baylyi (61); P, essential in Pseudomonas aeruginosa (118); E, essential in Escherichia coli (11); D, dispensable gene.
f
—, not applicable, no structure determined, or not present in Acinetobacter spp.