TABLE 6.

Lipoate metabolism proteins in protozoans^a

Organism	PDH	KDH	BCDH	GCV	Lipoylation enzymesb
Apicomplexa
Plasmodium falciparum 3D7	E1α, AAN35840; E1β, AAN37054; E2, AAN35399; E3, CAD51214	E1, CAD51167; E2, CAD52355; E3, AAN36396	E1α, CAD52260; E1β, CAD51411; E2, CAB38991	T, CAD52774; H, AAN35923	L, CAD52290; L2, CAD51918c; B, CAD51137; A, CAD52569
Toxoplasma gondii ME49	E1α, EEA99856; E1β, EEA98721; E2, EEB00702; E3, EEB03230	E1, EEA99768; E2, EEB03569; E3, EEB00688; ?, EEB02066d	E1α, EEA99447; E1α, EEB01412; E1β, EEA97499; E2, EEB02719	T, EEB01949; H, EEB00438	L, EEA98687; L2, EEB03119c; B, EEA97602; A, EEA99152
Cryptosporidium parvum Iowa II
Kinetoplastea
Trypanosoma brucei TREU927	E1α, EAN78647; E1β, AAZ10214; E2, EAN78163; E2, EAN77661e; E3, EAN80618; E3, AAZ10472; E3, AAZ11094; E3, AAZ13501	E1, EAN79950; E1, EAN79124; E2, EAN80119	E1α, EAN77496; E1β, EAN77853; E2, AAZ11544	T, EAN79919; H, EAN76953; P, AAZ12256	L, AAZ12834; B, EAN79891; A, EAN78865
Trypanosoma cruzi CL Brener	E1α, EAN92220 E and EAN93231 nE; E1β, EAN89795 E and EAN97324 nE; E2, EAN98042 E and EAN93711 nE; E2, EAN97566e; E3, EAN96941 E and EAN90443 nE; E3, EAN82368 E and EAN82673 nE; E3, EAN86891 E and EAN84842 nE; E3, EAN86892 E and EAN84843 nE	E1, EAN83551 E and EAN93176 nE; E1, EAN81565 E and EAN85332 nE; E2, EAN86475 E and EAN81897 nE; E2, EAN83551 E and EAN93176 nE	E1α, EAN89773 E and EAN86288 nE; E1β, EAN90241 E and EAN91015 nE; E2, EAN88993 E and EAN90623 nE	T, EAN86200 E and EAN85387 nE; H, EAN99694 E and EAN83079 nE; P, EAN91112f and EAN85656f	L, EAN85875 E and EAN92947 nE; B, EAN91274 E and EAN92615 nE; A, EAN97594 E and EAN90088 nE
Leishmania major Friedlin	E1α, CAJ04196; E1β, CAJ05399; E2, CAJ09224; E2, CAJ04084e; E3, CAJ08862; E3, AAZ09641; E3, CAJ08475; E3, CAJ08476	E1, CAJ09310; E1, AAZ09834; E2, CAJ05698	E1α, CAJ04806; E1β, AAZ14234; E2, CAJ01934	T, CAJ09346g; T, CAJ09347g; H, AAZ14696; P, CAJ04455	L, CAJ07073; B, CAJ09270; A, CAJ07120
Metamonada and Amoebozoa
Entamoeba histolytica HM-1:IMSS
Trichomonas vaginalis G3				H, EAX86583; H, EAY05732; L, EAY01593	L, EAX90813
Giardia lamblia ATCC 50803

^aProteins known or predicted to be located in the apicoplast are in bold. Others are thought to be located in the mitochondria or hydrogenosomes. Esmeralda (E) and nonesmeralda (nE) haplotype genes in Trypanosoma cruzi CL Brener are ∼98% identical.

^bLipoylation enzymes with homology to E. coli LplA (L), LipB (B), and LipA (A) are shown.

^cHighly divergent lipoate ligase enzymes referred to as LipL2 in the text. T. gondii EEB03119 should probably be annotated similarly to NCLIV_001780 from Neospora caninum.

^dThis protein is composed primarily of three lipoylation/biotinylation domains, the second of which resembles those found in KDH E2 subunits.

^eUnusually short PDH E2 paralogs conserved in kinetoplastid parasites.

^fSimilar genes from unidentified haplotypes.

^gDuplicated genes arrayed in tandem.