TABLE 2.

Thermocycle conditions used in PCR and sequencing reactions

Target	Reaction	Conditions			No. of cycles
		Denaturation	Annealing	Extension
porA	PCR	94°C, 1 min	68°C, 1 min	72°C, 2 min	30
	Seqa	96°C, 40 s	50°C, 40 s	60°C, 4 min	40
fHbp	PCR	96°C, 30 s	63°C, 30 s	72°C, 1 min	30
	Seq	96°C, 10 s	53°C, 5 s	60°C, 4 min	25
gna2132	PCR	96°C, 30 s	57°C, 30 s	72°C, 1 min	30
	Seq	96°C, 10 s	57°C, 5 s	60°C, 4 min	25
nadA	PCR	96°C, 30 s	56°C, 30 s	68°C, 1 min 20 s	30
	PCRb	96°C, 30 s	70°C, 30 s	72°C, 1 min 20 s	33
	Seq	96°C, 10 s	52°C, 5 s	60°C, 4 min	25

^aSeq, sequencing.

^bConditions for PCR using internally directed PCR primers.